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Determination of Isohumulones using Standard On-Line SPE Be the first to rate this application

Description

The BeerNHop solution using an on-line SPE-HPLC method was applied for the analysis of the Thermo Scientific isohumulones standard. Automated analyte enrichment was done by solid phase extraction (SPE) on a Thermo Scientific Hypersil GOLD C8 column. Then, the separation was performed on a Hypersil GOLD 3 μm column with UV detection at 270 nm. With a total run time of 15 minutes, the method allows fast screening for isohumulones in beer. Hence, beer bitterness is quantified and the isohumulone pattern is identified within minutes.
Market: Food and Beverage
Keywords: Beer, BeerNHop, Bitterness, Brewery, Brewing, Hop, iso-a-acids, Isohumulones
Matrix: Certified isohumulones standard in methanol (100 µg/mL)
Uploaded on 10/29/2014.

For Research Use Only. Not for use in diagnostic procedures.