The analysis of testosterone in human serum for clinical research is currently performed using many different sample preparation procedures. In this note, we evaluated a Prelude SPLC system coupled to a TSQ Endura MS (Figure 1) in the analysis of testosterone using two sample preparation methods: online sample cleanup and offline sample cleanup. Each method uses specific chromatography, but both share the same mass spectrometer settings and parameters.

The Thermo Scientific UltiMate 3000 HPLC system is applied for the determination of doxorubicin in human serum. The samples are prepared using Thermo Scientific SOLA solid phase extraction (SPE) cartridges, and the separation performed on a Thermo Scientific Hypersil GOLD HPLC column, with fluorescence detection. For Research Use Only. Not for use in diagnostic procedures.

The Thermo Scientific Dionex UltiMate 3000 RSLC system is applied for the analysis of Docetaxel in human serum. The sample was prepared using SOLA HRP solid phase extraction (SPE) 96 well plates, and the separation performed on a Thermo Scientific Accucore RP-MS HPLC column in less than 3 minutes, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.

The Thermo Scientific Dionex UltiMate 3000 HPLC system is applied for the analysis of Zolmitriptan in human serum. The sample was prepared using SOLA CX solid phase extraction (SPE) cartridges, and the separation performed on a Thermo Scientific Hypersil GOLD HPLC column in less than 2.5 minutes, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of Paclitaxel from human serum. The sample was prepared using SOLA HRP solid phase extraction (SPE), and the separation performed on a Thermo Scientific Accucore RP-MS HPLC column in less than 3 minutes, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.