CAN123: Sugars in Honey Using HPAE-PAD: What Is the Best Column?

Instrument Type: IC

Honey is defined by the Codex Alimentarius as the natural, sweet substance produced by honey bees from the nectar of plants or from secretions of living parts of plants or excretions of plant sucking insects on the living parts of plants, which the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in the honeycomb to ripen and mature. High-performance anion-exchange chromatography with pulsed amperometric detection is commonly used to determine the carbohydrates in honey. This document evaluates CarboPac columns for that purpose.

TN125: Guidelines for Successful Use of Thermo Scientific Dionex AminoTrap Columns

Instrument Type: IC

Free amino acids and small peptides can interfere with HPAE-PAD monosaccharide analysis. An AminoTrap column in front of a CarboPac column can remove the aforementioned interference. This TN describes how to use the AminoTrap and how to test if it functioning correctly. The record reports an AminoTrap test method.

AN82: Analysis of Fruit Juices Adulterated with Medium Invert Sugar from Beets (Method C)

Instrument Type: IC

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection(HPAE-PAD) discovered several components in beet medium invert sugar(BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, especially for oligosaccharides, the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required.

AN82: Analysis of Fruit Juices Adulterated with Medium Invert Sugar from Beets (Method A)

Instrument Type: IC

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) have discovered several components in beet medium invert sugar (BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, for oligosaccharides, and the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required.