This work develops methods for the determination of carbohydrates in wood acid hydrolysates using a CarboPac SA10-4μm column, along with a 0.4 µL injection and thicker working electrode gasket. This column contains smaller resin particles, enabling high-resolution, high-efficiency separation, particularly for the rhamnose-galactose that has been challenging to resolve with previous methods. The described method is fast, provides good sensitivity and consistent response, and can be routinely used to determine sugars in wood samples. An ICS-6000 can be used for this application.

This work develops methods for the determination of carbohydrates in wood acid hydrolysates using the CarboPac SA10-4μm column, along with a 0.4 µL injection and thicker working electrode gasket. This column contains smaller resin particles, enabling high-resolution, high-efficiency separation, particularly for rhamnose-galactose that has been challenging to resolve with previous methods. The described method is fast, provides good sensitivity and consistent response. An ICS-6000 can be used for this application.

This work updates the column used in AN1161 with a shorter 4 × 150 mm format column. The combination of the smaller resin particle size of the Dionex CarboPac SA10-4μm column and the shorter column used here results in separation of eight common sugars in less than six minutes. This is significant time savings as compared to the eight-minute runtime achieved in AN1089. The shorter run time allows for faster sample turnaround times and reduced eluent consumption, thereby improving the overall process economics. Results for method linearity, accuracy, and robustness are presented here.

The Accelerated Solvent Extraction (ASE) provides a fast and efficient way to extract various biomass samples for sugar analysis to determine sample viability for alcohol production. This application note describes methods for the extraction of sugars from biomass samples using acidified solvents (in-cell hydrolysis) and extracting pre-hydrolyzed samples.

Organic acids in crude oil, fuel, etc., are one of the biggest concerns of the oil, oil refining, and car manufacturing industries. Because of their corrosive properties and the associated costs of corrosion, organic acids have become a topic of great industrial interest. Ion chromatography (IC) can be used for the determination of low molecular weight organic acids in aqueous or water-miscible matrices. This application note summarizes the use and evaluation of an automated IC method for the direct determination of acetate and formate in diesel, oil, and diesel/oil mixtures.