We demonstrate a complete analytical metabolomic workflow including (1) data acquisition using a high resolution accurate mass instrument that is equipped with a Higher Energy Collisional Dissociation (HCD) cell and coupled to a high pressure LC (Figure 1), (2) metabolite differential abundance analysis, and (3) structural elucidation of relevant metabolites using accurate mass and HCD fragmentation information to highlight the component differences between green and black tea.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of catechins. The separation is performed on six different stationary phases; Thermo Scientific Acclaim C30, Acclaim Mixed-Mode HILIC-1, Acclaim Phenyl-1, Acclaim 120 C18, Acclaim PolarAdvanage and Acclaim PolarAdvantage II. The results were compared.

The Thermo Scientific Dionex UltiMate 3000 RSLC system is applied for the analysis of catechins. Catechins found in black and green tea are separated in less than 15 minutes using the Thermo Scientific Acclaim PA2 3 µm column with UV detection at 280 nm.

The Thermo Scientific Dionex UltiMate 3000 RSLC system is applied for the analysis of catechins. The separation of ten catechins was performed in 30 minutes on a Thermo Scientific Acclaim PA2 3 µm column with UV detection at 280 nm.

The Thermo Scientific HPLC system is applied for the analysis of catechins. The separation is performed on a Thermo Scientific Hypersil GOLD Phenyl HPLC column, with UV detection at 280 nm.