Clinical research labs commonly use selective and cost-efficient LC-MS techniques for the analysis of immunosupressants drugs. Usually, the quantitative method is developed on a triple quadrupole mass spectrometer. Here, we evaluated a high-efficiency method implemented on a Q Exactive Focus hybrid quadrupole Orbitrap mass spectrometer for improved selectivity when compared to the conventional solution.

Cannabis is the most frequently abused drug and THC the major psychoactive constituent. THC is primarily metabolized to THC-OH, which has equipotent psychoactivity and is further metabolized to nonpsychoactive THC-COOH. Second-phase metabolites, are also present in blood and can be used as markers to determine recency of intake while improving interpretation of analytical results. LC-MS analytical methods are widely used for analysis of THC and metabolites. LC-MS methods do not require sample derivatization, thus yielding savings over typical GC-MS procedures. For forensic use only.

We developed a a rapid, sensitive, selective, and robust LC-MS/MS method to determine the concentrations of cyclosporine A, tacrolimus, sirolimus, and everolimus in whole blood.

In this work, the performance of the Thermo Scientific TSQ Duo system is assessed for the quantitative analysis of 11 FAMEs in human blood spot extracts. The TSQ Duo system offers analysts a unique choice by allowing the use of single quadrupole methods and enabling the automatic transition to triple quadrupole on the same system. This application note illustrates the stepwise process of upgrading methods from single quadrupole to triple quadrupole using Chromeleon CDS software.

The determination of transition metals in physiological fluids is of considerable interest in clinical chemistry. This application note describes an attractive alternative to traditional spectroscopic methods by using the principles of ion exchange. Separation between individual metals can be enhanced or altered simply by changing eluents (pyridine-2, 6-dicarboxylic acid or oxalic acid). This figure illustrates the selectivity on an IonPac CS5A column when using a pyridine-2, 6-dicarboxylic acid eluent.