Demonstration of the advantages of using the Thermo Scientific Accucore Vanquish C18+, 1.5 μm column and Thermo Scientific Vanquish UHPLC system for the fast analysis of statins. The advanced capabilities of the Vanquish UHPLC system allow the Accucore Vanquish columns to be operated at high flow rates that enable development of rapid analytical methods while maintaining high performance.

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of atorvastatin impurities. The separation was performed on a Thermo Scientific Accucore aQ HPLC column using a reversed-phase method with UV detection at 254 nm.

The Thermo Scientific Dionex Ultimate 3000 system is applied for the related substance analysis of atorvastatin and ezetimibe. The separation is performed on a Thermo Scientific Accucore PFP HPLC column, with UV detection at 260 nm.

The Thermo Scientific Dionex UltiMate 3000 system is applied for the reaction monitoring separation of avorvastatin and its impurities. The separation was performed on a Thermo Scientific Accucore PFP column, with UV detection at 248 nm and a total run time of 18 minutes.

The Thermo Scientific HPLC system is applied for the analysis of atorvastatin. The separation is performed on a Thermo Scientific Hypersil GOLD Kappa column, with UV detection at 254 nm.