Acesulfame potassium, saccharin, aspartame, and sucralose are all commonly used artificial sweeteners that have been approved for use by the United States Food and Drug Administration (FDA). Sodium cyclamate has been banned in the United States, although it is approved for use in many other countries, including the UK. We demonstrate the use of the Thermo Scientific Accucore RP-MS HPLC column for the rapid analysis of five artificial sweeteners.

The Thermo Scientific HPLC system is applied for the analysis of soft drink ingredients. The separation is performed using a Thermo Scientific Acclaim Organic Acid (OA) column, with UV detection at 210 nm. The Thermo Scientific Acclaim OA column is uniquely suited because of its aqueous compatibility for the separation of organic acids, and its hydrophobic selectivity for the separation of preservatives.

Three formats of the Thermo Scientific Acclaim 120 C18 column are used for the analysis of aspartame and its impurities, with UV detection at 254nm.

The Thermo Scientific Acclaim Mixed-Mode WAX-1 column is used for the rapid analysis of soft drink ingredients, with UV detection at 210 nm. Due to the columns multi-functional chemistry, this assay can be performed in just 3 minutes.

The Thermo Scientific HPLC system is applied for the analysis of five artificial sweeteners. The separation was performed on a Thermo Scientific Accucore RP-MS HPLC column in under one minute, with MS/MS detection.