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Displaying 1-3 of 3 results for Tag: Adenosine

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AN1075: Ion Chromatography Assay for Chloride and Sulfate in Adenosine

Instrument Type: IC

Adenosine is produced by the condensation of ribose and adenine biochemically or by fermentation. Anions such as chloride and sulfate are possible impurities in adenosine preparations and the U.S. Pharmacopeia (USP) adenosine monograph describes turbidity-based assays to determine the amount of chloride and sulfate in adenosine which are subjective, use toxic chemicals, and generate hazardous waste. This application is an fast ion chromatography (IC)-based method that can meet the assay requirements and replace the USP adenosine monograph’s existing turbidity-based assays.

AN1072: Ion Chromatography Assay for Ammonia in Adenosine

Instrument Type: IC

Adenosine is produced by the condensation of ribose and adenine biochemically or by fermentation. Ammonia is an impurity in adenosine preparations and the U.S. Pharmacopeia (USP) adenosine monograph describes an assay to determine the amount of ammonia in adenosine by comparing the color of an adenosine sample and a ammonium chloride standard solution after the addition of an alkaline mercuric/potassium iodide solution to each. This application is an ion chromatography (IC)-based method that can meet the assay requirements and replace the USP adenosine monograph’s color-based method.

Rapid analysis of RNA nucleosides using a Thermo Scientific Accucore 150-Amide-HILIC column

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000RS system is applied for the analysis of RNA nucleosides. The separation is performed on a Thermo Scientific Accucore 150-Amide-HILIC column, with UV detection at 265 nm.