A rapid HPLC method using a Thermo Fisher Scientific Dionex Ultimate 3000 HPLC system comparing three detection technologies, ECD, UV and FLD, was developed for the simultaneous measurement of curcumin (C), demethoxycucurmin (DMC) and bisdemethoxycurcumin (CDMC). Curcuminoids in three food products, including turmeric power, curry powder and a pellet curry sauce, were quantitatively determined after simple ultrasonic extraction with methanol. Separation of curcuminoids was achieved in just 3 min using a Thermo Fisher Scientific Acclaim PA2 column.
The Thermo Scientific UltiMate 3000 HPLC system is applied for the separation of curcuminoids in turmeric. The separation is performed on a Thermo Scientific Accucore Polar Premium column, with UV detection at 428 nm. The separation achieved is compared to that obtained on a competitor C18 core-shell column.
The Thermo Scientific Acclaim PolarAdvantage II (PA2) 3 µm column is used to separate the three major yellow pigments known as curcuminoids in turmeric in less than 2.5 minutes.
The Thermo Scientific Accucore Polar Premium HPLC column has a polar-embedded C18 surface on a superficially porous silica substrate. This chemistry provides a selectivity that resolves the major and minor components of turmeric under simple isocratic conditions in about three minutes, while C18 columns fail to separate these components.
