The current work describes two methods that use a Dionex CarboPac PA20-4µm column for determination key sugars present in acid hydrolyzed algal biomass. The long method separates the two uronic acids in addition to the twelve other sugars separated by the shorter method. When a detailed sugar composition calculation is desired, the long method can be used. The method was validated for precision, accuracy, and robustness during routine use.
The current work describes two methods that use a Dionex CarboPac PA20-4µm column for determination key sugars present in acid hydrolyzed algal biomass. The short method separates twelve common carbohydrate sugars in less than fifteen minutes. This method does not separate uronic acids, which are key components that are present in low concentrations. The method was validated for precision, accuracy, and robustness during routine use.
This work develops methods for the determination of carbohydrates in wood acid hydrolysates using a CarboPac SA10-4μm column, along with a 0.4 µL injection and thicker working electrode gasket. This column contains smaller resin particles, enabling high-resolution, high-efficiency separation, particularly for the rhamnose-galactose that has been challenging to resolve with previous methods. The described method is fast, provides good sensitivity and consistent response, and can be routinely used to determine sugars in wood samples. An ICS-6000 can be used for this application.
This work develops methods for the determination of carbohydrates in wood acid hydrolysates using the CarboPac SA10-4μm column, along with a 0.4 µL injection and thicker working electrode gasket. This column contains smaller resin particles, enabling high-resolution, high-efficiency separation, particularly for rhamnose-galactose that has been challenging to resolve with previous methods. The described method is fast, provides good sensitivity and consistent response. An ICS-6000 can be used for this application.
This work updates the column used in AN1161 with a shorter 4 × 150 mm format column. The combination of the smaller resin particle size of the Dionex CarboPac SA10-4μm column and the shorter column used here results in separation of eight common sugars in less than six minutes. This is significant time savings as compared to the eight-minute runtime achieved in AN1089. The shorter run time allows for faster sample turnaround times and reduced eluent consumption, thereby improving the overall process economics. Results for method linearity, accuracy, and robustness are presented here.
