In this paper, A method for simultaneous determination of vitamin A, vitamin D2, vitamin D3 and vitamin E isomers by two-dimensional liquid chromatography was developed using Thermofisher Vanquish Core liquid chromatography platform. The saponification solution was purified by online solid phase extraction. This method has high sensitivity and good repeatability, and can be used for simultaneous determination of vitamin content in food containing the above-mentioned vitamins.
Vitamin D, including vitamin D2 and D3 (Figure 1), is essential to calcium absorption and bone health. Current studies suggest vitamin D could help prevent a wide range of disorders. Although vitamin D can be synthesized by the human body from exposure to sunlight, fortification in some dietary sources becomes necessary. We describe an easy and comprehensive LC-MS/MS method using a Transcend TLX-1 system powered by Thermo Scientific TurboFlow technology to measure vitamin D levels in infant formula. TurboFlow technology eliminates most traditional sample preparation steps.
The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of fat-soluble vitamins. The separation is performed using a Thermo Scientific Acclaim Phenyl-1 column, with UV detection at 220 nm.
The Thermo Scientific HPLC system is applied for the analysis of fat-soluble vitamin standards. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection at 385 nm.
The Thermo Scientific Ultimate 3000 RS system is applied for the analysis of vitamin D tablets. The separation was performed on the Thermo Scientific Accucore XL C18 column with a run time of 10 minutes.
