Solid phase extraction cleans up wines reproducibly and reliably, facilitating analysis of underivatized amino acids in wines. Mass detection enables the quantification of co-eluting amino acids, in comparison with method involving derivatization and UV or FL detection.
Implementation of a flow injection analysis-tandem mass spectrometry (FIA-MS/MS) method for simultaneous quantification of 13 amino acids and 13 acylcarnitines in dried blood spots. Newborn screening (NBS) is a public health program provided by most of the countries around the world aimed at screening newborns for a list of serious genetic and metabolic disorders.1-4 Determination of the amino acids and acylcarnitines profile is an integral part of the newborn screening programs.
Development of a sensitive and reproducible method for analysis of 22 underivatized amino acids and application of the method to quantify 17 amino acids in wine. Accurate quantification of proline, using an isotopically labeled internal standard.
The goal of this application is to analyze the non-proteinogenic amino acid carbocisteine and its impurities using HPLC-CAD (Charged Aerosol Detection) and to obtain improved sensitivity for the impurities when compared to the existing method.
We developed a flow injection analysis-tandem mass spectrometry (FIA-MS/MS) research methods for simultaneous detection and quantification of 12 amino acids, 18 acylcarnitines, and succinylacetone in dried blood spots and compare derivatization and nonderivatization sample preparation methods.
