Optimization and validation of a multi-residue LC-MS/MS method for pesticides in spinach and cabbage by using Thermo Scientific Vanquish UHPLC and TSQ Quantis mass spectrometer. Sample preparation was performed using QuEChERS method that provided high recoveries for an extensive scope of pesticides in several simple steps. Data acquisition and processing were carried out using Thermo Scientific TraceFinder software. The optimized method was validated as per the SANTE guideline and applied to real samples demonstrating that the workflow is fit for purpose.
We present a multi-residue instrumental method that can be applied for high-throughput screening and semi-quantitation of pesticide residues in food matrices at or below the current legislative requirements. A high-resolution, accurate-mass mass spectrometer operated in Full Scan – Variable Data-Independent Analysis (FS-vDIA)* mode provided an option for full spectrum filtering, retrospective analysis, and multi-parameter-based compound identification. The method was validated for 328 target pesticides, with an option for the future extension to a larger number. (*vDIA not available in US.)
The Thermo Scientific™ TSQ™ 8000 Evo triple quadrupole GC-MS is an excellent tool for the control of MRL levels in food commodities. The enhanced velocity optics (EVO) driving EvoCell collision cell technology provide high SRM transition speeds, precision, and sensitivity for even complex methods involving several pesticides in a short run time. This application is focused on the analysis of 247 compounds in two different herbal juices, Aloe vera and Amla (Indian gooseberry), demonstrating the potential of the method to detect trace level compounds at concentrations as low as 1-2 ng/g.
Monitoring for pesticide and other chemical residues in produce is essential to maintaining a safe food supply. Monitoring is typically done by public agencies, but budget restrictions have increased pressure on these agencies to improve productivity while lowering costs. We demonstrate the ability of a high-resolution, accurate-mass UHPLC-MS system, combined with appropriate application-specific workflow software, to provide fast, confident, and precise screening and quantitative analysis of pesticides in onion matrix.
We developed a quick and efficient LC-MS/MS method for screening pesticides in orange oil and ginseng extract using TraceFinder software.
