Worldwide food safety concerns have risen dramatically as the number of food contamination incidents and product recalls has increased. Every food analysis starts with sample preparation, which is widely accepted as one of the most critical steps of LC/MS. Increased demand for higher throughput, accuracy and lower matrix interference from food analysis laboratories has made sample preparation the largest bottleneck. Turbulent flow chromatography technology can eliminate the need for lengthy offline sample preparation steps, thereby eliminating these disadvantages.
Most current methodologies for the quantitation of antibiotics in drinking water revolve around analysis using triple stage quadrupole platforms with offline sample preparation. This is a proven technique for the analysis of many contaminants in various types of water samples, but the offline sample preparation steps are time-consuming and prone to operator error and reproducibility problems. We demonstrate the ability to directly inject the water sample without any offline preconcentration. We utilize LC-MS with a Thermo Scientific Exactive Plus Orbitrap MS using HR/AM.
Veterinary drugs are pharmacologically active compounds that can result in non-desirable drug residues in the food chain. Animals with high amounts of these drugs can cause harm in humans or can render the use of antibiotics useless due to the counter effects of the drugs. The quantification of over 200 multi-class veterinary drugs from different meat products usually involves time consuming extraction methods with either SPE or LLE. We describe a robust method, utilizing a single LC-MS/MS run to quantitate over 200 veterinary drugs in a variety of matrices.
There is increasing demand to provide rapid and selective screening techniques for a wide range of antibiotic drugs. Their abuse as a feed supplement in food animals is of growing concern and is subject to regulations globally. Creating screening methods for multiple analytes is more cost effective than dedicated methods for fewer analytes. This application shows the advantages of using the Thermo Scientific Accucore Vanquish C18 UHPLC 1.5 µm column and Vanquish UHPLC system for the analysis of thirty-six antibiotics.
There is growing environmental concern regarding the health impact of trace levels of pharmaceuticals and personal care products (PPCPs) in water resources. In response, the U.S. Environmental Protection Agency (EPA) published Method 1694, which determines dozens of PPCPs in water, soil, sediment, and biosolids by HPLC-MS/MS. We demonstrate a progressive approach to analyzing PPCPs in environmental sources of water at the ng/L level with online sample preparation using small volumes of water, thus saving time and reducing the cost of analysis.
