This application note describes a convenient, fast, and sensitive method that uses a Thermo Scientific™ Dionex™ CarboPac™ PA300 column and electrolytic eluent generation. The Dionex CarboPac PA300-4μm column is packed with a new 4 μm particle size supermacroporous resin to achieve high efficiency and high-resolution separations. Using this column, a 16.8 min method that resolved six analytes, including glycol and other carbohydrates, was designed. Results for method linearity, robustness, and accuracy for glycol and carbohydrate quantification in OTC samples are discussed here.
To demonstrate that the Norma Oficial Mexicana (Official Mexican Standard) method for agave syrup carbohydrate analysis can be executed with a Thermo Scientific™ Dionex™ CarboPac™ PA200-1 mm column using a KOH/KMSA eluent produced electrolytically using dual eluent generation cartridge (Dual EGC) mode
In this application note, we describe a new method involving eluent generation and high pressure ion chromatography with 4 μm particle size resin for sorbitol determination in fruits and beverages. This methodology provides faster results and improves reliability with perfect and automatic control of eluent concentration.
This application demonstrates that the Norma Oficial Mexicana (Official Mexican Standard) method for agave syrup carbohydrate analysis can be executed with a Thermo Scientific™ Dionex™ CarboPac™ PA1-1mm column using KOH/KMSA eluent produced electrolytically using Dual Eluent Generation Cartridge (Dual EGC) mode.
In this application note, the NOM method was evaluated with a Dionex CarboPac PA1 250 X 2 mm column. The 2mm column requires a flow rate approximately 4 time less than the 4-mm column, and thus reduces eluent consumption. Key performance parameters were evaluated including separation, linearity, limits of detection, and precision. Three samples were analyzed and the main sugars, polyol, and HMF in those sample were determined. Inositol was also determined because it was reported to be present in agave syrup. Adulteration was evaluated with amyloglucosidase enzymatic hydrolysis.
