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AN73132: Carbohydrate Analysis of Agave Syrup using HPAE-PAD
Description
In this application note, the NOM method was evaluated with a Dionex CarboPac PA1 250 X 2 mm column. The 2mm column requires a flow rate approximately 4 time less than the 4-mm column, and thus reduces eluent consumption. Key performance parameters were evaluated including separation, linearity, limits of detection, and precision. Three samples were analyzed and the main sugars, polyol, and HMF in those sample were determined. Inositol was also determined because it was reported to be present in agave syrup. Adulteration was evaluated with amyloglucosidase enzymatic hydrolysis.| Market: | Food and Beverage |
| Keywords: | carbohydrate, Electrochemical detection, fructan, oligosaccharides, Adulteration, PAD, Dionex ICS 5000+ HPIC system, Dionex ICS-6000 HPIC system, TN71, Agave Syrup, Dionex CarboPac PA1-2mm column, polyols, Mexico NOM, AN 73132, TN 20, TN 70671, TN20, TN70671 |
| Matrix: | Agave Syrup |
| Author: | Jingli Hu and Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
