The method transfer between the Thermo Scientific™ UltiMate™ 3000 UHPLC system and a Thermo Scientific™ Vanquish™ Flex UHPLC system is seamless and, in this case, for an isocratic separation requires no method redevelopment. The Thermo Scientific™ Acclaim™ Mixed Mode WAX-1 column proved capable of the analysis of neutral and ionic compounds that would be otherwise poorly retained on solely reversed-phase columns.The %RSD of peak area was found to be at least 50% lower for the majority of compounds analysed using the Thermo Scientific Vanquish Flex systems proprietary autosampler technology.
The analysis of food additives in carbonated beverages is important as many additives are controlled as part of the formulations of these beverages. In addition, analysis may detect possible counterfeiting of branded products. Being able to identify and quantify food additives in beverages quickly and with high sensitivity is therefore important.
The Thermo Scientific Acclaim Mixed-Mode WAX-1 column is used for the rapid analysis of soft drink ingredients, with UV detection at 210 nm. Due to the columns multi-functional chemistry, this assay can be performed in just 3 minutes.
The Thermo Scientific Acclaim Mixed-Mode WAX-1 column is used for the separation of ingredients in a toothache pain-reliever, with UV detection at 210 nm.
In this work, the baseline separation of three preservatives (sorbate, benzoate and benzisothiazolone) was achieved with excellent peak efficiency and peak shape with the Thermo Scientific Acclaim Mixed-Mode WAX-1 column.
