A robust, sensitive, and reliable multi-residue LC-MS/MS method workflow solution that uses the Thermo Scientific TSQ Altis triple quadrupole mass spectrometer provides analysis and quantitation of more than 160 veterinary drugs with the Thermo Scientific VetDrugs Explorer Collection solution in salmon (fillet), bovine muscle, and milk. The complete instrument and data processing method including SRM settings is included with the VetDrugs Explorer Collection start-to-finish workflow solution. In 17 minutes, the target veterinary drugs were detected and quantified in food matrices.
Forty-four multi-class veterinary drugs of known concentrations were employed to demonstrate how the use of a generic variable data-independent acquisition (vDIA) method with wide MS/MS precursor isolation windows achieves sensitivity and selectivity comparable to data-dependent MS2 acquisition (using narrow isolation windows) in quantitative and qualitative small molecule applications. In addition, a full record of MS and MS/MS data for the measured sample fit for non-targeted and unknown screening purposes is delivered. vDIA is not available in the United States of America
To describe a new method for veterinary drug analysis, showing how the variable data-independent acquisition (vDIA) workflow achieves high sensitivity and selectivity, providing a complete high-quality data record of the measured sample. Quantitative analysis of the acquired data in combination with non-targeted and unknown screening is shown. vDIA is not available in the United States of America
Chromeleon 7.2 CDS supports a quantitative method for targeted analysis of antibiotics with LC coupled to both UV and MS detection. The application is realized with state-of-the-art LC-MS instrumentations. The Thermo Scientific Vanquish UHPLC system is coupled to the Thermo Scientific Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometer. Here we present step-by-step the creation of the instrument method, data processing method, and report template, that are thereafter used to generate an LC-MS eWorkflow.
The Thermo Scientific Acclaim Trinity P2 provides baseline separation of both penicillin G and potassium ion with excellent resolution and good peak shape using a simple isocratic method.
