To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
• To demonstrate the capability of the Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) and Thermo Scientific™ Vanquish™ Flex Binary UHPLC system combination for the rapid separation of water soluble vitamins in sports beverages with excellent linearity, reproducibility, resolution, and recoveries. • To show the capability of the Vanquish Flex Binary UHPLC system to provide superior retention time precision through its novel pump features.
The Thermo Scientific Hypersil GOLD HILIC column was used to separate water soluble vitamins using UV detection at 205, 230 and 260 nm in under 8 minutes.
The Thermo Scientific Dionex UltiMate 3000 system is applied for the analysis of nicotinic acid and thiocyanate. The separation was performed on a Thermo Scientific Acclaim Mixed-Mode WAX-1 column, with UV detection at 210 nm.
