The Thermo Scientific™ Vanquish™ Flex UHPLC system is applied for the Related substance analysis of Caspofungin. The analysis was performed on the Thermofisher Scientific Acclaim C30 HPLC Column. The results obtained for the Resolution and theoretical plates met the criteria.
Like the majority of pharmacopeial LC methods, the chromatographic analysis of temozolomide and its four related impurities given by European and United States Pharmacopeia relies on reversed-phase separation. However, the analytes are very hydrophilic, and the method is based on highly aqueous mobile phase and ion-pairing reagents. Thus, hydrophilic interaction liquid chromatography (HILIC) appears as a convenient alternative. In the current work, a new HILIC method was set up and was successfully transferred from an Agilent 1260 Infinity LC (1260 Infinity) system to a Vanquish Core HPLC.
Reliable and highly reproducible chromatographic results were obtained when implementing the liquid chromatography-based impurity method from the Ph. Eur. monograph of metolazone.
Simple stability-indicating LC-MS method for erythromycin. Compare the impurity profiles of an erythromycin reference standard with stressed analyte.
The presented results show that Thermo Scientific™ Vanquish™ Core HPLC systems have excellent system-to system reproducibility when the sample preparation, eluent preparation, and LC column are identical. Users can rely on the fact that the performance of multiple Vanquish Core HPLC systems will be highly predictable and robust for routine methods in quality control labs when other variables are controlled.
