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Setup and transfer of a gradient HILIC method for the impurity analysis of temozolomide
Description
Like the majority of pharmacopeial LC methods, the chromatographic analysis of temozolomide and its four related impurities given by European and United States Pharmacopeia relies on reversed-phase separation. However, the analytes are very hydrophilic, and the method is based on highly aqueous mobile phase and ion-pairing reagents. Thus, hydrophilic interaction liquid chromatography (HILIC) appears as a convenient alternative. In the current work, a new HILIC method was set up and was successfully transferred from an Agilent 1260 Infinity LC (1260 Infinity) system to a Vanquish Core HPLC.| Market: | Pharma |
| Keywords: | HILIC, USP, HPLC method transfer, Vanquish Core HPLC system, Agilent 1260 Infinity LC system, Ph. Eur., temozolomide |
| Matrix: | Acetonitrile |
| Author: | Maria Grübner |
| Affiliation: | Thermo Fisher Scientific, Germering, Germany |
