We recently introduced a PdH RE for HPAE-PAD carbohydrate determinations. In this work, we demonstrate two popular carbohydrate applications (honey sugars and glycoprotein monosaccharides) using a PdH RE and compare the results to using an Ag/AgCl RE. Our preliminary data indicate that the PdH RE provides comparable results to the standard Ag/AgCl RE for these two applications that use hydroxide eluents.
The increased market demand for lactose-free products has created a need for a fast, reliable, and sensitive method to analyze these products. HPAE-PAD is one of the most powerful techniques for carbohydrate determinations. It is a well-established technique for lactose and lactulose determinations. In this work, we developed an HPAE-PAD method using the recently introduced Thermo Scientific™ Dionex™ CarboPac™ PA210-4µm column to separate lactose from other structurally similar milk sugars. Here we determined lactose in eleven lactose-free products (both dairy and baked) using HPAE-PAD.
The current work describes two methods that use a Dionex CarboPac PA20-4µm column for determination key sugars present in acid hydrolyzed algal biomass. The long method separates the two uronic acids in addition to the twelve other sugars separated by the shorter method. When a detailed sugar composition calculation is desired, the long method can be used. The method was validated for precision, accuracy, and robustness during routine use.
The current work describes two methods that use a Dionex CarboPac PA20-4µm column for determination key sugars present in acid hydrolyzed algal biomass. The short method separates twelve common carbohydrate sugars in less than fifteen minutes. This method does not separate uronic acids, which are key components that are present in low concentrations. The method was validated for precision, accuracy, and robustness during routine use.
This record describes method for reproduction of column Quality Assurance Report (QAR). Analyzing a standard sample using method conditions described in QAR is recommended in order to validate column performance prior to use. This record also includes a one-click eWorkflow for executing the QAR method on a ICS 5000+ system.
