This application note illustrates the use of an easy-to-implement IC-MS method for the determination of aliphatic and unsaturated organic acids in pharmaceutical solutions. This workflow employs both suppressed conductivity and MS detection to increase the information available from each sample. Samples were separated on a high-resolution Dionex™ IonPac™ AS11-HC-4μm column set using a Dionex™ Integrion™ HPIC™ system with suppressed conductivity detection. The sequential MS detection was achieved using a Thermo Scientific™ ISQ™ EC single quadrupole mass spectrometer.
This report demonstrates the proof of concept to determine eight nucleotides and six organic acid metabolites by anion-exchange chromatography with suppressed conductivity detection. The metabolites were separated on the 2 mm i.d. Thermo Scientific Dionex IonPac AS11-HC-4um column, using electrolytically generated KOH gradient. These experiments were facilitated by the Thermo Scientific Dionex ICS-6000 HPIC system. Compare these results against those using methanol organic modifier (another record in AppsLab and in the attached report).
This report demonstrates the proof of concept to determine eight nucleotides and six organic acid metabolites by anion-exchange chromatography with suppressed conductivity detection. The metabolites were separated on the 2 mm i.d. Thermo Scientific Dionex IonPac AS11-HC-4um column, using electrolytically generated KOH gradient with methanol as the organic modifier to resolve malate and succinate. These experiments were facilitated by the Thermo Scientific Dionex ICS-6000 HPIC system.
Before running any samples, Thermo Scientific recommends that you first confirm the performance of the column by reproducing the lot validation report chromatogram shipped with column.Compare your results with the one reported in the quality assurance report. At least three injections should be made. This record provides an eWorkflow for executing the QAR method on an ICS-5000 system.<enter final 90 characters of description>
This application note describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds of interest to the brewing industry, including: carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. One of two forms of electrochemical detection is used, pulsed amperometry or conductivity detection. This part of the application note describes organic acid analysis which uses conductivity detection. This was formerly Dionex AN46.
