Thermo Scientific AppsLab Library: Separation of Eight Nucleotides and Six Organic Acids: KOH-Methanol Gradient

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Description

This report demonstrates the proof of concept to determine eight nucleotides and six organic acid metabolites by anion-exchange chromatography with suppressed conductivity detection. The metabolites were separated on the 2 mm i.d. Thermo Scientific Dionex IonPac AS11-HC-4um column, using electrolytically generated KOH gradient with methanol as the organic modifier to resolve malate and succinate. These experiments were facilitated by the Thermo Scientific Dionex ICS-6000 HPIC system.

Market:	Metabolomics
Keywords:	AMP, ATP, Formate, Malate, Nucleotides, Sugar phosphates, dTTP, Citrate, Succinate, Isocitrate, Metabolites, RFIC, Oxalate, Reagent-Free IC, NADPH, metabolomics, ICS-6000, TCA cycle, IonPac AS11HC, KOH methanol gradient, methanol KOH gradient, methanol modifier, phospho-sugars, dCMP, NAD, dGMP, UTP, ADRS 600, AN1137, AN622
Matrix:	Standards in DI Water
Author:	Linda Lin and Michael Hvizd
Affiliation:	Thermo Fisher Scientific
Status:	Proof of Concept

Uploaded on 7/24/2019.

For Research Use Only. Not for use in diagnostic procedures.