The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Size exclusion analysis of Rituximab. The separation was performed on a Thermo Scientific MabPac SEC-1 column with a UV detection at 220 nm. With a run time of 30 minutes, the method allows easy separation of Aggregates and Dimer of Rituximab
Size exclusion chromatography (SEC) is one of the major analyses in biotherapeutics characterization. Here we show the separation of a mAb (monomer) and its aggregates (e.g., dimer) with the Thermo Scientific Vanquish Flex UHPLC system. Produced from biological cell cultures, mAbs may contain significant amounts of dimers, trimers and other higher order aggregates. The MAbPac SEC-1 is specially designed for analysis of mAbs and their aggregates.
The Thermo Scientific HPLC system is applied for the analysis of propafenone and related substances. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 249 nm.
The Thermo Scientific HPLC system is applied for the determination of acrylic acid oligomers. The separation is performed on a Thermo Scientific Organic Acid HPLC column, with UV detection at 210 nm.
