This application shows the quantification of multiple impurities with a single calibrant by taking advantage of the uniform response of the charged aerosol detector (CAD). The inverse gradient compensation wizard is also highlighted.
This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.
To demonstrate the use of a multi-pump UHPLC system and enable tandem analysis with two columns in parallel, addressing productivity and throughput improvement of existing LC-MS methods.
Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish Flex UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on an Thermos Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions allow reliable qualitative and quantitative data interpretation.
The Thermo Scientific Vanquish UHPLC system was used in combination with the Thermo Scientific Acclaim RSLC 120 C18, 2.2 µm, 2.1x250 mm column to provide high resolution peptide separation of a monoclonal antibody digest. The column was thermostatted at 40, 60, and 80 °C. Both still-air and forced-air mode were used. The highest peak capacity was obtained at 80 °C, and with still-air thermostatting mode. At 40 °C, the peak capacity obtained with still-air and forced-air was comparable.
