This work aimed to develop and validate an analytical method for simultaneous screening and quantification of pesticide residues in potato by using the QuEChERS extraction method in combination with the Thermo Scientific™ Exactive™ GC Orbitrap™ GC-MS system operated in full scan mode. The data acquisition and processing were carried out by using Thermo Scientific™ TraceFinder™ software. The optimized method was validated as per the SANTE/12682/2019 guidelines.
Optimization and validation of a multi-residue LC-MS/MS method for pesticides in spinach and cabbage by using Thermo Scientific Vanquish UHPLC and TSQ Quantis mass spectrometer. Sample preparation was performed using QuEChERS method that provided high recoveries for an extensive scope of pesticides in several simple steps. Data acquisition and processing were carried out using Thermo Scientific TraceFinder software. The optimized method was validated as per the SANTE guideline and applied to real samples demonstrating that the workflow is fit for purpose.
We present a multi-residue instrumental method that can be applied for high-throughput screening and semi-quantitation of pesticide residues in food matrices at or below the current legislative requirements. A high-resolution, accurate-mass mass spectrometer operated in Full Scan – Variable Data-Independent Analysis (FS-vDIA)* mode provided an option for full spectrum filtering, retrospective analysis, and multi-parameter-based compound identification. The method was validated for 328 target pesticides, with an option for the future extension to a larger number. (*vDIA not available in US.)
As agricultural trade grows and food safety concerns mount, stricter pesticide regulations are being enforced around the world. Increased pesticide testing and reductions in maximum permissible residue levels have driven demand for fast, sensitive and cost-effective analytical methods for high-throughput screening of multi-class pesticides in food. Detection of 510 pesticides at low ppb levels was achieved within 12 minutes using the Thermo Scientific Exactive benchtop LC/MS system powered by Orbitrap technology.
European Regulation 396/2005 sets maximum residue levels of pesticides in different products of plant and animal origin. These regulations present a significant analytical challenge with respect to LOQs required for some specified food matrices such as baby food. The multi-residue pesticide method described can be applied to fruits, cereals and composite baby foods at LODs in the range of 0.8–10.3 µg/kg - below respective EU maximum residue limits (MRLs). The method is validated for 48 pesticides from different classes, but can be readily extended to a larger number of residues.
