This application note illustrates the use of an easy-to-implement IC-MS method for the determination of aliphatic and unsaturated organic acids in pharmaceutical solutions. This workflow employs both suppressed conductivity and MS detection to increase the information available from each sample. Samples were separated on a high-resolution Dionex™ IonPac™ AS11-HC-4μm column set using a Dionex™ Integrion™ HPIC™ system with suppressed conductivity detection. The sequential MS detection was achieved using a Thermo Scientific™ ISQ™ EC single quadrupole mass spectrometer.
Before running any samples, Thermo Scientific recommends that you first confirm the performance of the column by reproducing the lot validation report chromatogram shipped with column.Compare your results with the one reported in the quality assurance report. At least three injections should be made. This record provides an eWorkflow for executing the QAR method on an ICS-5000 system.<enter final 90 characters of description>
This application note describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds of interest to the brewing industry, including: carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. One of two forms of electrochemical detection is used, pulsed amperometry or conductivity detection. This part of the application note describes organic acid analysis which uses conductivity detection. This was formerly Dionex AN46.
The Thermo Scientific HPLC system is applied for the analysis of glycolic and related acids. The separation is performed on a Thermo Scientific Acclaim Organic Acid (OA) column, with UV detection at 210 nm.
This study shows a method for determining eight organic acids in wastewater using ion-exclusion chromatography and on-line carbonate removal. Normally, carbonate and inorganic anions are present in wastewater samples and interfere in organic acid analysis using ion exchange chromatography. In this study, the interferences are eliminated using ion-exclusion chromatography. Inorganic anions are eluted in the void volume and carbonate is removed by an on-line carbonate removal device.
