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In-depth protein glycosylation comparison of a biosimilar candidate by hydrophilic interaction UHPLC Be the first to rate this application


The Thermo Scientific Vanquish H system with fluorescence detection is applied for the glycan profile comparison of a biosimilar candidate produced ‘in house’ using Chinese hamster ovary (CHO) cell line using HILIC-based chromatographic separation on Accucore 150-Amide-HILIC column. Developed methodology offers an excellent analytical platform for glycan profile comparison of multiples lots of the biosimilar product.
Market: BioPharma
Keywords: Biotherapeutics, Glycosylation, IgG, N-glycans, Monoclonal Antibodies, Trastuzumab, Innovator, Biosimilar, NIBRT, Bioprocessing Research
Matrix: Biosimilar product
Author: Silvia Millán, Anne Trappe, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Status: Proof of Concept
Uploaded on 12/20/2016.

For Research Use Only. Not for use in diagnostic procedures.