In-depth protein glycosylation comparison of a biosimilar candidate by hydrophilic interaction UHPLC
DescriptionThe Thermo Scientific Vanquish H system with fluorescence detection is applied for the glycan profile comparison of a biosimilar candidate produced ‘in house’ using Chinese hamster ovary (CHO) cell line using HILIC-based chromatographic separation on Accucore 150-Amide-HILIC column. Developed methodology offers an excellent analytical platform for glycan profile comparison of multiples lots of the biosimilar product.
|Keywords:||Biotherapeutics, Glycosylation, IgG, N-glycans, Monoclonal Antibodies, Trastuzumab, Innovator, Biosimilar, NIBRT, Bioprocessing Research|
|Author:||Silvia Millán, Anne Trappe, Amy Farrell and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
|Status:||Proof of Concept|
For Research Use Only. Not for use in diagnostic procedures.