USP 38 monograph: assay analysis of tetracycline hydrochloride using a C8 HPLC column
DescriptionThe Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay analysis of tetracycline hydrochloride. The separation was performed on a Thermo Scientific Acclaim 120 C8 HPLC column using the method described in the USP 38 monograph. The results obtained exceeded the criteria stated in the USP. Resolution between 4-epianhydrotetracycline hydrochloride and tetracycline hydrochloride peak was 3.5 (USP criteria of not less than 1.2). Relative retention time of 4-epianhydrotetracycline hydrochloride with respect to tetracycline hydrochloride peak was 0.82 (USP criteria about 0.9).
|Keywords:||Acclaim 120 C8, HPLC, UV detection, USP 38 monograph, Tetracycline hydrochloride, 4-epianhydrotetracycline hydrochloride|
|Affiliation:||Thermo Fisher Scientific|
For Research Use Only. Not for use in diagnostic procedures.