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USP 38 monograph: assay analysis of tetracycline hydrochloride using a C8 HPLC column Be the first to rate this application


The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay analysis of tetracycline hydrochloride. The separation was performed on a Thermo Scientific Acclaim 120 C8 HPLC column using the method described in the USP 38 monograph. The results obtained exceeded the criteria stated in the USP. Resolution between 4-epianhydrotetracycline hydrochloride and tetracycline hydrochloride peak was 3.5 (USP criteria of not less than 1.2). Relative retention time of 4-epianhydrotetracycline hydrochloride with respect to tetracycline hydrochloride peak was 0.82 (USP criteria about 0.9).
Market: Pharma
Keywords: Acclaim 120 C8, HPLC, UV detection, USP 38 monograph, Tetracycline hydrochloride, 4-epianhydrotetracycline hydrochloride
Matrix: N/A
Author: Manish Singh
Affiliation: Thermo Fisher Scientific
Uploaded on 6/30/2015.

For Research Use Only. Not for use in diagnostic procedures.