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Single Peak Quantitation of Tween 20 by HPLC and Corona Charged Aerosol Detection
Description
Tween 20, or polysorbate 20, is a complex non-ionic surfactant which is quantified using a fast gradient HPLC method on a C1 column. The universal, mass-based Corona charged aerosol detector is used for quantitation of this polymeric analyte. Baseline subtraction is used to eliminate baseline effects. Limit of quantitation is 20 ng on column (o.c.), based on a peak area relative standard deviation (RSD) of 15%. The dynamic range was 9.8 - 5000 ng o.c. with R^2 of 0.9993 and a calibration RSD of 4.4%. This method is designed for a fast quantification of Tween 20 as a raw material.| Market: | BioPharma |
| Keywords: | Polysorbate 20, single peak, Tween 20 |
| Matrix: | Water |
| Author: | Marc Plante |
For Research Use Only. Not for use in diagnostic procedures.
