Single Peak Quantitation of Tween 20 by HPLC and Corona Charged Aerosol Detection

Instrument Type: HPLC

Tween 20, or polysorbate 20, is a complex non-ionic surfactant which is quantified using a fast gradient HPLC method on a C1 column. The universal, mass-based Corona charged aerosol detector is used for quantitation of this polymeric analyte. Baseline subtraction is used to eliminate baseline effects. Limit of quantitation is 20 ng on column (o.c.), based on a peak area relative standard deviation (RSD) of 15%. The dynamic range was 9.8 - 5000 ng o.c. with R^2 of 0.9993 and a calibration RSD of 4.4%. This method is designed for a fast quantification of Tween 20 as a raw material.

Single Peak Quantitation of Tween 80 by HPLC and Corona Charged Aerosol Detection

Instrument Type: HPLC-CAD

Tween 80, or polysorbate 80, is a complex non-ionic surfactant which is quantified using a fast gradient HPLC method on a C1 column. The universal, mass-based Corona charged aerosol detector is used for quantitation of this polymeric analyte. Baseline subtraction is used to eliminate baseline effects. Limit of quantitation is 78 ng on column (o.c.), based on a peak area relative standard deviation (RSD) of 15%. The dynamic range was 78 - 5000 ng o.c. with R^2 of 0.9997 and a calibration RSD of 2.8%. This method is designed for a fast quantification of Tween 80 as a raw material.