Rapid analysis of natural sweeteners found in food and beverages using an advanced UHPLC system

Instrument Type: UHPLC

Over the last decade there has been a growing interest in low-calorie alternatives to carbohydrate-based sweeteners. Recent publications have shown a dramatic increase in attention toward natural extracts such as the Stevia rebaudiana plant, not only for its sweetening effect but also for additional health benefits attributed to the plant. The major sweetening components are stevioside, rebaudioside A, rebaudioside C, and dulcoside A, each of which is over 300 times sweeter than sucrose based sweeteners. Because of this they are widely used in beverages and foodstuffs.

Determination of Steviosides by HPLC-CAD on UltiMate 3000

Instrument Type: HPLC-CAD

This application combines the Acclaim Trinity P1 with charged aerosol and diode array detection to quantify stevioside sweeteners used in foods and beverages. The two detectors are complimentary at higher concentrations of steviosides, but under the conditions used here the charged aerosol detector provides superior sensitivity for most of the analytes.