Evaluation of Prelude SPLC and TSQ Endura Mass Spectrometer in Research Analysis of Testosterone in Human Serum

Instrument Type: LCMSMS

The analysis of testosterone in human serum for clinical research is currently performed using many different sample preparation procedures. In this note, we evaluated a Prelude SPLC system coupled to a TSQ Endura MS (Figure 1) in the analysis of testosterone using two sample preparation methods: online sample cleanup and offline sample cleanup. Each method uses specific chromatography, but both share the same mass spectrometer settings and parameters.

Quantitative Forensic Analysis of Opiates, Opioids, and Their Metabolites in Human Urine Without Hydrolysis

Instrument Type: LCMSMS

Analysis of opiate and opioid metabolites in urine is most often done with a hydrolysis step that make total sample preparation time up to 24 hours. The method described here eliminates the hydrolysis step by analyzing the conjugated metabolites intact using a Thermo Scientific Prelude SPLC system for sample preparation and a Thermo Scientific TSQ Endura triple quadrupole mass spectrometer for analysis. For forensic use only.