AN1149: Profiling Fructosyloligosaccharides (FOS)-containing samples by HPAE-PAD

Instrument Type: IC

In this work, the oligo- and polysaccharide distribution of three different inulin samples has been characterized by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to determine FOS content in prebiotic samples and variation in prebiotics on addition of probiotics. We used Inulin-FOS and chicory root inulin as prebiotic samples. For probiotics, we used Yakult drink, a well known probiotic dairy product. In this method, separation of individual oligosaccharides in FOS was achieved on a Dionex CarboPac PA200 column. An ICS-6000 can be used for this application.

AN67: Determination of Plant-Derived Neutral Oligo- and Polysaccharides

Instrument Type: IC

This application note describes how to develop an HPAE-PAD method for separating plant-derived neutral oligo- and polysaccharides. Amylopectin and fructan separations demonstrate column, eluent, and gradient choices for developing rugged HPAE-PAD methods for neutral oligo- and polysaccharides.