Rapid and sensitive UHPLC screening of food dyes in carbonated beverages using UV/Vis wavelength switching

Instrument Type: UHPLC

The analysis of food dyes in carbonated beverages is important as many of these dyes are either controlled substances or are being phased out in certain countries due to their reported adverse health effects. Being able to identify and quantify food dyes in beverages quickly and with high sensitivity is therefore important. Reversed-phase chromatography is an excellent technique for the analysis of dyes. Many dyes are readily soluble in reversed-phase eluents and have strong visible and UV absorbance properties. This method demonstrates the separation of ten contentious dyes.

Rapid analysis of yellow dye in breakfast cereal using a Thermo Scientific Acclaim 120 C18 HPLC column

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of yellow dye in breakfast cereal. The separation is performed on a Thermo Scientific Acclaim 120 C18 HPLC column, with UV detection at 427, 508 and 625 nm.