The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay analysis of phytonadione. The analysis was performed on a Thermo Scientific Hypersil GOLD Silica HPLC column using the method described in the USP 39 monograph. The results obtained for resolution met the criteria stated in the USP. The results obtained for resolution between the peaks due to (Z)-phytonadione and (E)-phytonadione was 2.1 (USP criteria of NLT 1.5).

Vitamin K1 isomers and related compounds are separated using a silica HPLC column and a normal phase solvent system. The correct silica type and mobile phase equilibration are important considerations in order to achieve good resolution and retention time reproducibility.

The Thermo Scientific HPLC system is applied for the analysis of vitamin K1 and K2. The separation is performed on a Thermo Scientific Acclaim HILIC-10 column, with UV detection at 265 nm.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of fat-soluble vitamins. The separation is performed using a Thermo Scientific Acclaim Phenyl-1 column, with UV detection at 220 nm.

The Thermo Scientific HPLC system is applied for the analysis of fat-soluble vitamin standards. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection at 385 nm.