Sensitive and Accurate Quantitation of Perfluorinated Compounds in Human Breast Milk using Selected Reaction Monitoring Assays by LC/MS/MS

Instrument Type: LCMSMS

Perfluorinated compounds (PFCs) are ubiquitous and persistent pollutants that bioaccumulate in animals and humans. The potential toxicity of these chemicals has fueled efforts to develop robust analytical techniques for measuring low levels of PFCs in human matrices. We describe a quantitative selected reaction monitoring (SRM) assay developed for six PFCs using the Thermo Scientific TSQ Vantage triple-stage quadrupole mass spectrometer (MS) coupled to a PFC-free Thermo Scientific Accela LC system.

Validated Method for the Determination of Phenylbutazone in Horse Meat with LC-MS/MS

Instrument Type: LCMSMS

Phenylbutazone (bute, PBT) is one of the most widely used (non-steroidal) anti-inflammatory (NSAiD) and painkiller drug for dogs and horses. Horse meat is still widely used for human consumption in some European countries. For those animals marked for human consumption, a limited number of medicines can be administered. A newly developed LC-MS/MS method for the determination of PBT in horse meat with LC-MS/MS is described here. The method was validated in-house and its applicability for routine testing was tested following the European legislation requirements.