Quantitation of THC and THC Metabolites in Blood Using SOLAµ SPE Plates and the TSQ Quantiva Triple Quadrupole Mass Spectrometer for Forensic Analysis

Instrument Type: LCMSMS

Cannabis is the most frequently abused drug and THC the major psychoactive constituent. THC is primarily metabolized to THC-OH, which has equipotent psychoactivity and is further metabolized to nonpsychoactive THC-COOH. Second-phase metabolites, are also present in blood and can be used as markers to determine recency of intake while improving interpretation of analytical results. LC-MS analytical methods are widely used for analysis of THC and metabolites. LC-MS methods do not require sample derivatization, thus yielding savings over typical GC-MS procedures. For forensic use only.

A Sensitive and Efficient Method to Analyze THC and THCCOOH in Oral Fluid Using LC-MS/MS in Forensic Toxicology Laboratories

Instrument Type: LCMSMS

The Substance Abuse and Mental Health Services Administration (SAMHSA) lists delta9-tetrahydrocannabinol (THC) as the only target analyte for detection of cannabis use in oral fluid at 2 ng/mL confirmation cutoff concentration. In this study, we developed a sensitive and efficient method for simultaneous determination of THC and THCCOOH in OF. Both analytes were extracted using a simple and fast SPE method without pre-conditioning, evaporation, or reconstitution. THCCOOH was detected with high sensitivity (LOQ 10 pg/mL), and no derivatization procedure was required. For forensic use only.