Determination of High Sugar Concentrations in a Flavored Rum Liquor Sample Using a Compact Ion Chromatography System

Instrument Type: IC

This record demonstrates the determinations of glucose, fructose, and sucrose in a 100-fold diluted flavored rum alcohol sample by HPAE-PAD. Typically samples with g/L concentrations require > 10,000-fold dilutions to remain in the linear range of the very sensitivie HPAE-PAD technique. However, this method, demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.

Rum Analysis using HPAE-PAD

Instrument Type: IC

This is an e-workflow for rum analysis using HPAE-PAD. The method descried here uses Dionex CarboPac PA20 column to separate residual sugars in commercial rum samples. A faster analysis may be possible on the Dionex CarboPac SA10 column. This was a quick feasibility study and we believe the AminoTrap guard column used here can be replaced by the Dionex CarboPac PA20 guard. Rum samples were diluted 100-fold with DI water before analysis.