Full-Scan Fragmentation Options for the Detection of Food Contaminants by an Affordable LC-Q-Orbitrap MS

Instrument Type: LCMS

The analysis of food toxicants is challenging because of the high number of substances that need to be analyzed. Pesticides alone account for over 800 analytes including other types of toxicants such as mycotoxins, plant toxins and/or veterinary drugs. The use of liquid chromatography with full-scan, high-resolution accurate mass spectrometry (HRAM) as an alternative is gaining in popularity. We compare two different scan options of a quadrupole-Orbitrap system, both offering full mass range fragmentation techniques, and to optimize performance in terms of sensitivity and selectivity

Variable Data-Independent Acquisition (vDIA) Delivers High Selectivity and Sensitivity in Combined Targeted and Untargeted Analyses for Small Molecules

Instrument Type: LCMSMS

Forty-four multi-class veterinary drugs of known concentrations were employed to demonstrate how the use of a generic variable data-independent acquisition (vDIA) method with wide MS/MS precursor isolation windows achieves sensitivity and selectivity comparable to data-dependent MS2 acquisition (using narrow isolation windows) in quantitative and qualitative small molecule applications. In addition, a full record of MS and MS/MS data for the measured sample fit for non-targeted and unknown screening purposes is delivered. vDIA is not available in the United States of America