Detection of Mycotoxins in Corn Meal Extract Using Automated Online Sample Preparation with LC-MS/MS

Instrument Type: LCMSMS

Methyl 2-benzimidazole carbamate, most commonly known as carbendazim, is a widely used broad-spectrum benzimidazole fungicide and a decomposition product of benomyl. Carbendazim is used to control plant diseases in cereals and fruit, including citrus, bananas, strawberries, pineapples, and pome fruits. We describe a method that is an adaptation of an existing online, multi-residue pesticide method (Thermo Scientific Method 522133) proven and verified specially for the actual carbendazim contamination issue of orange juices in the US.

Determination of Fusarium Mycotoxins in Wheat, Maize and Animal Feed Using an Online TurboFlow and Orbitrap LC/MS Method

Instrument Type: LCMSMS

We describe a method using the Thermo Scientific Transcend TLX system with TurboFlow technology, which combines chromatography with high-resolution mass spectrometry, was developed and validated in house for the determination of deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, fumonisins B1 and B2 in maize (corn), wheat and animal feed. Linearity range, limit of detection (LOD), limit of quantification (LOQ), recovery, repeatability, intermediate precision and accuracy were established by analysis using certified reference materials and the performance of successful proficiency testing.

Determination of Multiple Mycotoxins in Grain Using a QuEChERS Sample Preparation Approach and LC-MS/MS Detection

Instrument Type: LCMSMS

Mycotoxins are toxic secondary metabolites that are naturally produced by several species of fungi on agricultural products, particularly grain-based products. We demonstrate a fast, easy, and cost-effective approach for the determination of 16 mycotoxin residues in grain-based food using QuEChERS sample preparation with a Thermo Scientific Accucore aQ HPLC column and a Thermo Scientific TSQ Vantage triple quadrupole mass spectrometer for HPLC separation.

High-Throughput Food Safety Control Employing Real Time Ionization (DART) Coupled to Orbitrap High-Resolution Mass Spectrometry

Instrument Type: Unspecified

This application note shows the possibilities using the DART ion source coupled with the ultra high-resolution Thermo Scientific Exactive mass spectrometer for rapid detection and quantitation of a wide range of food contaminants like mycotoxins and food adulterants (melamine).