EP 8.0 monograph: impurity determination of metronidazole using a C18 HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of metronidazole and its impurities. The separation was performed on a Thermo Scientific Syncronis C18 HPLC column using the method described in the EP 8.0 monograph. The results obtained for resolution and tailing factor exceeded the criteria stated in the EP. Resolution between impurity A and metronidazole was 6.9 (EP criteria of not less than 2.0) and tailing factor for metronidazole was 1.0 (EP criteria not more than 2.0).

USP 38 monograph: impurity determination of metronidazole using a C8 HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Metronidazole and its impurities. The separation was performed on a Thermo Scientific Acclaim 120 C8 HPLC column using the method described in the USP 38 monograph. The results obtained for resolution and tailing factor exceeded the criteria stated in the USP. Resolution between tinidazole compound A and metronidazole was 8.1 (USP criteria of not less than 2.0) and tailing factor for metronidazole was 1.0 (USP criteria not more than 2.0).

Simple assay analysis of norfloxacin and metronidazole in formulation using reversed-phase HPLC-UV

Instrument Type: HPLC

The Thermo Scientific Dionex Ultimate 3000 system is applied for the assay analysis of norfloxacin and metronidazole in formulation using a simple reversed-phase isocratic method. The two compounds were separated using a Thermo Scientific Hypersil GOLD 5 µm HPLC column in less than 10 minutes with UV detection at 290 nm.