AN71410: Ion Chromatography: A Versatile Technique for the Analysis of Beer (Part B: Malto-oligosaccharides)

Instrument Type: IC

This application note describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds of interest to the brewing industry, including: carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. One of two forms of electrochemical detection is used, pulsed amperometry or conductivity detection.This part of the application note describes the malto-oligosaccharide method which uses pulsed amperometry detection. This was formerly Dionex AN46.

AN83: Size-Exclusion Chromatography of Polysaccharides with Pulsed Amperometric Detection (PAD).

Instrument Type: IC

Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. Size-exclusion columns separate biological macromolecules by hydrodynamic size. Pulsed amperometric detection (PAD), provides sensitive and specific detection of carbohydrates. Here, fractionated pullulan standards were used to determine the separation range of linear carbohydrates.This application note shows how SEC can be coupled to PAD.

AN71410: Ion Chromatography: A Versatile Technique for the Analysis of Beer (Part A: Carbohydrate Analysis)

Instrument Type: IC

This document describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds in beer, including carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. Although many of these constituents can be determined individually using unrelated analytical techniques, reduced analysis time and equipment costs can be realized by using one instrument with multi-species capability. This part of the work deals with the carbohydrate determination which uses pulsed amperometry determination. This was formerly Dionex AN46.