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AN123: Determination of Inorganic Anions and Organic Acids in Fermentation Broths

Instrument Type: IC

Fermentation broths are complex mixtures of nutrients, waste products, cells and cell debris, and desired products. Fermentation monitoring is important for many industries because carbon sources and metabolic by-products can impact the yield of the desired products. This application note describes the use of two different anion-exchange columns, IonPac AS11 and IonPac AS11-HC, with suppressed conductivity detection, to analyze common organic and inorganic anions in yeast and bacterial fermentation broths.

AN107: Ions (Anions) in Physiological Fluids

Instrument Type: IC

One of the most important applications of IC in clinical chemistry is the analysis of physiological fluids such as urine, plasma, and serum both for inorganic and organic anions and cations. This application note outlines the analysis of physiological fluids using ion chromatography. Part one shows the anion method, the IonPac AS11 is coupled with the Anion Self-Regenerating Suppressor (ASRS) to dramatically reduce eluent conductivity, thus allowing the use of a rapidly increasing hydroxide gradient for the separation of an extensive group of monovalent through trivalent.

AN1107: Determination of Anions and Carboxylic Acids in Urban Fine Particles (PM2.5)

Instrument Type: IC

Ionic forms of air pollutants are analyzed here using anion-exchange chromatography and suppressed conductivity. The gas sample included particles < 2.5 um (PM2.5) was collected using ambient ion monitoring and then dissolved in deionized water. The dissolved inorganic anions and organic acids were separated on a 4 mm i.d. IonPac AS11-HC column and detected by suppressed conductivity. This method provided analysis of more ionic contaminants than standard methods (EPA Method 26A) and ASTM D5085-02). An ICS-6000 can be used for this application.

AN206: Determination of Oxalate and Other Anions in Bayer Liquor Using Ion Chromatography

Instrument Type: IC

A direct injection method was designed using a Reagent‑Free™ Ion Chromatography (RFIC™) system with in-line sample cleanup to accurately and reproducibly measure oxalate in Bayer liquor. The online sample preparation removes most of the aluminum so that it does not contaminate either the column or suppressor. Fluoride is used to promote ligand exchange with oxalate for aluminum. This online sample preparation method allows an accurate, reproducible, and rugged determination of oxalate in Bayer liquor.