The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Lactulose. The separation was performed on a Thermo Scientific Hypersil Gold amino HPLC column using the method as described in the USP 38 monograph. Resolution between Epilactose and Lactose Peak was 1.28 (met USP criteria of NLT 0.9), RRT for Epilactose with respect to Lactulose peak was 0.92 (met USP criteria of about 0.83), Resolution between Lactulose and Lactose Peak was 3.17 (met USP criteria of NLT 1.5), RRT for Lactose with respect to Lactulose peak was 1.22 (met USP criteria of about 1.1),

The second method in this application note takes advantage of the selectivity and high capacity of the Dionex CarboPac MA1 column. Use of this column allows mannitol to be strongly retained with minimal interferences. These advantages have led to many publications that can be found in the reference section of the application note.

Certain carbohydrates such as mannitol, rhamnose, xylose, 3-O-methylglucose, and lactulose can be determined in urine and serum samples to evaluate disease states in animals and humans. These carbohydrates can be determined directly by HPAE-PAD, a well-established technique that eliminates the need for derivitization, in turn saving time and money. In this work, the separation and detection of the same carbohydrates of interest discussed in AN 1006 are demonstrated using a capillary Dionex CarboPac PA20 anion-exchange column on the ICS-4000 Dedicated Capillary HPIC system.

This work describes a sensitive and accurate method to extract, separate, and quantify lactose and lactulose in milk-based products. The method uses a Dionex CarboPac PA20 column with PAD to quantify lactose and lactulose in a separation time of less than 30 min. The use of disposable gold electrodes provides the benefit of high electrode-to-electrode reproducibility and rapid equilibration upon installation.

This application demonstrates fast separations of lactose and lactulose in milk products by HPAE-PAD on the 4 mm Dionex CarboPac SA10-4μm column, which was facilitated by the high-pressure capable Dionex ICS-5000+ HPIC system. An ICS-6000 can be used for this application.