Essential Lipidomics Experiments Using the LTQ Orbitrap Hybrid Mass Spectrometer

Instrument Type: LCMSMS

We tested the performance of a novel mass spectrometer, the Thermo Scientific LTQ Orbitrap, in a multitude of different applications in the field of lipidomics. The experiments presented here take advantage of the LTQ Orbitrap capabilities of multiple stage fragmentation, high resolution and mass accuracy to solve challenges of quantitating isomeric glycerophospholipids, resolving isobaric species, and elucidating fragmentation pathways and compositional assignment of various lipid species.

Analysis of Beer Using a High Speed U-HPLC Coupled to a Linear Ion Trap Hybrid Mass Spectrometer

Instrument Type: LCMSMS

We coupled a HRAM instrument to an ultra high pressure LC. The optimized LC method decreased the total analysis time from 130 to 25 minutes. Considerable time and cost savings facilitate routine process monitoring and quality control applications. Reproducible chromatography combined with reliable highly accurate mass measurements on the LTQ Orbitrap enable fast profiling of complex mixtures for their comparison (typing). Also,unknown components of the mixtures can be identified and their chemical structures confidently assigned using the MSn fragmentation capabilities of the LTQ Orbitrap.