To describe a fast and reproducible optimization procedure for the isolation of opioids from human urine utilizing solid phase extraction (SPE). Subsequent analysis was carried out with liquid chromatography separation coupled to triple quadrupole mass spectrometry detection (LC-MS/MS).

The Thermo Scientific Dionex UltiMate 3000 LC system is applied to the estimation of Dicyandiamide. The analysis was performed on a Thermo Scientific Hypersil Gold aQ HPLC column with UV detection at 215 nm. With a total run time of 10 minutes the method allows rapid detection of Dicyandiamide.

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the detection of Sucralfate. The separation was performed on a Thermo Scientific Hypersil Gold aQ column. The linearity is established between concentrations of 250 ppm to 3000 ppm. The obtained regression for 6 different concentrations is 0.999. The LOD of the method is set for concentration of 250 ppm. The LOQ of the method is set as concentration of 500 ppm.

This application shows the advantages of using the Thermo Scientific™ Hypersil GOLD aQ VANQUISH™ 1.9 µm column and Thermo Scientific™ Vanquish™ UHPLC system, for the fast analysis of sulfur-containing drugs. The built-in method scaling of the Chromeleon software and high pressure capabilities of the Hypersil GOLD VANQUISH columns, that allow operation at higher flow rates,enable the development of rapid analytical methods whilst maintaining high performance.

The assay of hydroxyzine hydrochloride was performed on a Thermo Scientific Dionex UltiMate 3000 system with UV detection at 232 nm. The Thermo Scientific Hypersil GOLD aQ column was used to perform the assay with a run time of 25 minutes.