Related Compound analysis of Lactulose as per USP monograph

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Lactulose. The separation was performed on a Thermo Scientific Hypersil Gold amino HPLC column using the method as described in the USP 38 monograph. Resolution between Epilactose and Lactose Peak was 1.28 (met USP criteria of NLT 0.9), RRT for Epilactose with respect to Lactulose peak was 0.92 (met USP criteria of about 0.83), Resolution between Lactulose and Lactose Peak was 3.17 (met USP criteria of NLT 1.5), RRT for Lactose with respect to Lactulose peak was 1.22 (met USP criteria of about 1.1),

Simple determination of sorbitol using HPLC with RI detection

Instrument Type: HPLC

In this work the Thermo Scientific Hypersil GOLD Amino column was used for the determination of sorbitol. A simple isocratic method was used with RI detection and a run time of seven minutes was achieved.

HILIC determination of sugars by HPLC with RI detection

Instrument Type: HPLC

The separation of five sugars was performed using the Thermo Scientific Hypersil GOLD Amino column in HILIC mode with RI detection.